Caracterización taxonómica integradora y primer código de barras de ADN de Chrysozephyrus syla (Kollar, [1844]) y C. birupa (Moore, 1877) para especies indias (Lepidoptera: Lycaenidae)

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DOI:

https://doi.org/10.57065/shilap.1138

Palabras clave:

Lepidoptera, Lycaenidae, Theclini, Chrysozephyrus, código de barras de ADN, COI, genitalia, taxonomía integrativa, India

Resumen

El género Chrysozephyrus Shirôzu & Yamamoto, 1956 (tribu: Theclini) contiene especies con una gran plasticidad morfológica, lo que a menudo complica su identificación precisa. Este estudio ofrece una evaluación taxonómica integradora de C. syla (Kollar, [1844]) y C. birupa (Moore, 1877) basada en especímenes de la región de Himachal Pradesh, en el Himalaya indio. Cabe destacar que esta investigación ofrece la primera descripción formal e ilustración de la genitalia de la hembra de C. birupa, junto con las primeras ilustraciones completas de la genitalia del macho y de la hembra de C. syla. Para complementar estos hallazgos morfológicos, se generaron secuencias de citocromo c oxidasa I (COI) mitocondrial, lo que representa los primeros datos globales de códigos de barras de ADN para estas especies. El análisis molecular reveló un sesgo característico de A + T (70,3 % de media), en consonancia con la evolución mitocondrial de los Lepidoptera. La reconstrucción filogenética preliminar utilizando la máxima verosimilitud confirma la ubicación de estos taxones indios dentro del clado Theclini, mostrando bajas distancias genéticas intergenéricas (5,0-6,5 %) que se alinean con las hipótesis genómicas recientes que sugieren una posible sinonimia dentro del grupo Hypaurotis Scudder, 1876. Estos hallazgos proporcionan bases morfológicas y moleculares esenciales para los Lycaenidae raros de gran altitud y subrayan la importancia de integrar la morfología genital con herramientas moleculares para resolver la compleja sistemática de la tribu.

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Cantlie, K. (1963). The Lycaenidae portion (except the Arhopala group) of Brigadier Evans’ The Identification of Indian Butterflies 1932 (India, Pakistan, Ceylon, Burma). Bombay Natural History Society.

Dincă, V., Dapporto, L., Somervuo, P., Vodă, R., Cuvelier, S., Gascoigne-Pees, M., Huemer, P., Mutanen, M., Hebert, P. D., & Vila, R. (2021). High resolution DNA barcode library for European butterflies reveals continental patterns of mitochondrial genetic diversity. Communications Biology, 4(1), 315. https://doi.org/10.1038/s42003-021-01834-7 PMid:33750912 PMCid:PMC7943782 DOI: https://doi.org/10.1038/s42003-021-01834-7

Eliot, J. N. (1973). The higher classification of the Lycaenidae (Lepidoptera): a tentative arrangement. Bulletin of the British Museum (Natural History), Entomology, 28, 373-505. https://doi.org/10.5962/bhl.part.11171 DOI: https://doi.org/10.5962/bhl.part.11171

Evans, W. H. (1932). The identification of Indian butterflies (second ed. revised). Bombay Natural History Society.

Flomer, O. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular marine biology and biotechnology, 3, 294-299.

Fujioka, T. (1993). A list of Theclini of the world. In S Hurita. ed. Zephyrus. (in Japanese) Garlani, L. (2024). Annotated Checklist of Rhopalocera of Himachal Pradesh, India (Insecta: Lepidoptera). SHILAP Revista de lepidopterología, 52(208), 623-676. https://doi.org/10.57065/shilap.1009 DOI: https://doi.org/10.57065/shilap.1009

Hausmann, A., Haszprunar, G., Segerer, A. H., Speidel, W., Behounek, G., & Hebert, P. D. (2011). Now DNAbarcoded: the butterflies and larger moths of Germany. Spixiana, 34(1), 47-58.

Hebert, P. D., Cywinska, A., Ball, S. L., & DeWaard, J. R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences, 270(1512), 313-321. https://doi.org/10.1098/rspb.2002.2218 PMid:12614582 PMCid:PMC1691236 DOI: https://doi.org/10.1098/rspb.2002.2218

Hebert, P. D., Dewaard, J. R., Zakharov, E. V., Prosser, S. W., Sones, J. E., McKeown, J. T., & La Salle, J. (2013). A DNA ‘Barcode Blitz’: Rapid digitization and sequencing of a natural history collection. PloS one, 8(7), e68535. https://doi.org/10.1371/journal.pone.0068535 PMid:23874660 PMCid:PMC3707885 DOI: https://doi.org/10.1371/journal.pone.0068535

Howarth, T. G. (1957). A revision of the genus Neozephyrus Sibatani and Ito (Lepidoptera: Lycaenidae). Bulletin of the British Museum (Natural History), Entomology, 5, 233-285.

Huang, H. (2021). New or little-known butterflies from China-4. Atalanta, 52(3), 345-413.

Kehimkar, I. D. (2016). Butterflies of India: BNHS field guides. Bombay Natural History Society.

Kim, M. I., Wan, X., Kim, M. J., Jeong, H. C., Ahn, N. H., Kim, K. G., Han, Y. S., & Kim, I. (2010). Phylogenetic relationships of true butterflies (Lepidoptera: Papilionoidea) inferred from COI, 16S rRNA and EF-1α sequences. Molecules and cells, 30, 409-425. https://doi.org/10.1007/s10059-010-0141-9 PMid:20853063 DOI: https://doi.org/10.1007/s10059-010-0141-9

Klots, A. B. (1970). Lepidoptera. Taxonomist’s glossary of genitalia in insects (pp. 115-130). J. Jørgensen & CO. Koiwaya, S. (1999). A tentative list of the Theclini of the world. Nishikaze-Tsushin 10, 2-12.

Koiwaya, S. (2007). The Zephyrus hairstreaks of the World. Mushi-Sha [in Japanese]

Kokate, P. P., Techtmann, S. M., & Werner, T. (2021). Codon usage bias and dinucleotide preference in 29 Drosophila species. G3, 11(8), jkab191. https://doi.org/10.1093/g3journal/jkab191 PMid:34849812 PMCid:PMC8496323 DOI: https://doi.org/10.1093/g3journal/jkab191

Kumar, V., Kundu, S., Chakraborty, R., Sanyal, A., Raha, A., Sanyal, O., Ranjan, R., Pakrashi, A., Tyagi, K., & Chandra, K. (2019). DNA barcoding of Geometridae moths (Insecta: Lepidoptera): a preliminary effort from Namdapha National Park, Eastern Himalaya. Mitochondrial DNA Part B, 4(1), 309-315. https://doi.org/10.1080/23802359.2018.1544037 DOI: https://doi.org/10.1080/23802359.2018.1544037

Lavinia, P. D., Núñez Bustos, E. O., Kopuchian, C., Lijtmaer, D. A., Garcia, N. C., Hebert, P. D., & Tubaro, P. L. (2017). Barcoding the butterflies of southern South America: Species delimitation efficacy, cryptic diversity and geographic patterns of divergence. PloS one, 12(10), e0186845. https://doi.org/10.1371/journal.pone.0186845 PMid:29049373 PMCid:PMC5648246 DOI: https://doi.org/10.1371/journal.pone.0186845

Lomov, B., Keith, D. A., Britton, D. R., & Hochuli, D. F. (2006). Are butterflies and moths useful indicators for restoration monitoring? A pilot study in Sydney’s Cumberland Plain Woodland. Ecological Management & Restoration, 7(3), 204-210. https://doi.org/10.1111/j.1442-8903.2006.00310.x DOI: https://doi.org/10.1111/j.1442-8903.2006.00310.x

Lukhtanov, V. A., Sourakov, A., Zakharov, E. V., & Hebert, P. D. (2009). DNA barcoding Central Asian butterflies: increasing geographical dimension does not significantly reduce the success of species identification. Molecular ecology resources, 9(5), 1302-1310. https://doi.org/10.1111/j.1755-0998.2009.02577.x PMid:21564901 DOI: https://doi.org/10.1111/j.1755-0998.2009.02577.x

Nakabayashi, Y., & Ohshima, I. (2024). Geographical variation in parasitoid communities and the cause of enemyfree space in a range-expanding myrmecophilous lycaenid butterfly. Biological Journal of the Linnean Society, 141(1), 17-32. https://doi.org/10.1093/biolinnean/blad060 DOI: https://doi.org/10.1093/biolinnean/blad060

Näsvall, K., Boman, J., Talla, V., & Backström, N. (2023). Base composition, codon usage, and patterns of gene sequence evolution in butterflies. Genome Biology and Evolution, 15(8), evad150. https://doi.org/10.1093/gbe/evad150 PMid:37565492 PMCid:PMC10462419 DOI: https://doi.org/10.1093/gbe/evad150

Odagiri, K. (2004). Molecular phylogeny of the silvicolous hairstreaks, Favonius and Japonica (Lepidoptera: Lycaenidae) [PhD Thesis]. Kyushu University.

Prieto, C., Núñez, R., & Hausmann, A. (2019). Molecular species delimitation in the genus Rhamma Johnson, 1992 (Lepidoptera: Lycaenidae, Theclinae). Mitochondrial DNA Part A, 30(1), 101-117. https://doi.org/10.1080/24701394.2018.1462348 PMid:29671657 DOI: https://doi.org/10.1080/24701394.2018.1462348

Ratnasingham, S., & Hebert, P. D. (2013). A DNA-based registry for all animal species: the Barcode Index Number (BIN) system. PloS one, 8(7), e66213. https://doi.org/10.1371/journal.pone.0066213 PMid:23861743 PMCid:PMC3704603 DOI: https://doi.org/10.1371/journal.pone.0066213

Robinson, G. S. (1976). The preparation of slides of Lepidoptera genitalia with special reference to the Microlepidoptera. Entomologist’s Gazette, 27, 127-132.

Saito, K., & Hasegawa, T. (2016). Description of a new species of the subtribe Theclina (Lepidoptera: Lycaenidae) from Central Vietnam. Butterflies, 72, 46 48.

Shirôzu, T. (1956). A generic revision and the phylogeny of the tribe Theclini (Lepidoptera, Lycaenidae). Sieboldia, 4, 329-421.

Varshney, R. K., & Smetacek, P. (2015). A synoptic catalogue of the Butterflies of India. [1st ed.]. Butterfly Research Centre.

Wahlberg, N., Braby, M. F., Brower, A.V., De Jong, R., Lee, M. M., Nylin, S., Pierce, N. E., Sperling, F. A., Vila, R., Warren, A. D., & Zakharov, E. (2005). Synergistic effects of combining morphological and molecular data in resolving the phylogeny of butterflies and skippers. Proceedings of the Royal Society B: Biological Sciences, 72(1572), 1577-1586. https://doi.org/10.1098/rspb.2005.3124 PMid:16048773 PMCid:PMC1560179 DOI: https://doi.org/10.1098/rspb.2005.3124

Wiemers, M., & Fiedler, K. (2007). Does the DNA barcoding gap exist? a case study in blue butterflies (Lepidoptera: Lycaenidae). Frontiers in zoology, 4, 1-16. https://doi.org/10.1186/1742-9994-4-8 PMid:17343734 PMCid:PMC1838910 DOI: https://doi.org/10.1186/1742-9994-4-8

Wynter-Blyth, M. A. (1957). Butterflies of the Indian Region. Bombay Natural History Society.

Yang, F., Ding, F., Chen, H., He, M., Zhu, S., Ma, X., Jiang, L., & Li, H. (2018). DNA barcoding for the identification and authentication of animal species in traditional medicine. Evidence-Based Complementary and Alternative Medicine, 2018(1), p.5160254. https://doi.org/10.1155/2018/5160254 PMid:29849709 PMCid:PMC5937547 DOI: https://doi.org/10.1155/2018/5160254

Yang, M., Li, J., Su, S., Zhang, H., Wang, Z., Ding, W., & Li, L. (2021). The mitochondrial genomes of Tortricidae: nucleotide composition, gene variation and phylogenetic performance. BMC genomics, 22, 1-12. https://doi.org/10.1186/s12864-021-08041-y PMid:34674653 PMCid:PMC8532297 DOI: https://doi.org/10.1186/s12864-021-08041-y

Zhang, J., Cong, Q., Shen, J., Opler, P. A., & Grishin, N. V. (2020). Genomic evidence suggests further changes of butterfly names. The taxonomic report of the International Lepidoptera Survey, 8, 7.

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30-06-2026

Cómo citar

Chopra, D., Kaur Walia, G., & Kaur Sidhu, A. (2026). Caracterización taxonómica integradora y primer código de barras de ADN de Chrysozephyrus syla (Kollar, [1844]) y C. birupa (Moore, 1877) para especies indias (Lepidoptera: Lycaenidae). SHILAP Revista De lepidopterología, 54(214), 271–285. https://doi.org/10.57065/shilap.1138

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