Molecular identification of Trichogramma Westwood, 1833 species as egg parasitoids of Ostrinia nubilalis (Hübner, 1796) in corn production areas of Sakarya province in Türkiye (Insecta: Lepidoptera, Hymenoptera)
DOI:
https://doi.org/10.57065/shilap.1086Palavras-chave:
Insecta, Lepidoptera, Hymenoptera, ITS2, Trichogramma, Ostrinia, biological control, molecular systematics, TürkiyeResumo
The study focuses on using ITS2 sequences from rDNA for systematic studies of Trichogramma Westwood, 1833 species. ITS2 sequences have shown to be reliable in distinguishing Trichogramma species. Accurate identification of natural enemies is essential for biological control programs. The aim of the study is to compare rDNA-ITS2 sequences from Trichogramma samples with sequences in GenBank. By utilizing ITS2 as a barcode, the study aims to achieve dependable species identification and evaluate genetic diversity within Trichogramma species. ITS2 sequence was used to differentiate two Trichogramma species, Trichogramma euproctidis (Girault, 1911) and Trichogramma brassicae Bezdenko, 1968 collected from Sakarya province of Türkiye. The ITS2 sequences of the two Trichogramma species were aligned using Clustal W, and genetic distances as well as a phylogenetic tree were calculated using MEGA V7.0. In the study, rDNA-ITS2 sequences of fifty four Trichogramma specimens were confirmed in GenBank. Additionally Mfold web server was used to predict secondary structures of ITS2 sequences. The construction of all secondary structures was carried out at 37ºC using RNA version 2.3 default parameters. The study identified a new species that expands the Trichogramma fauna of Sakarya Province in northwestern Türkiye, where only one species had previously been detected through rDNA-ITS2 sequence analysis. This shows the importance of molecular markers in species identification and biological control strategies.
Downloads
Estatísticas globais ℹ️
|
2
Visualizações
|
0
Downloads
|
|
2
Total
|
|
Referências
Al-Barrak, M., Loxdale, H. D., Brookes, C. P., Dawah, H. A., Biron, D. G., & Alsagair, O. (2004). Molecular evidence using enzyme and RAPD markers for sympatric evolution in British species of Tetramesa (Hymenoptera: Eurytomidae). Biological Journal of the Linnean Society, 83, 509-525. https://doi.org/10.1111/j.1095-8312.2004.00408.x DOI: https://doi.org/10.1111/j.1095-8312.2004.00408.x
Chang, S. C., Hu, N. T., Hsin, C. Y., & Sun, C. N. (2001). Characterization of differences between two Trichogramma wasps by molecular markers. Biological Control, 21, 75-78. https://doi.org/10.1006/bcon.2001.0924 DOI: https://doi.org/10.1006/bcon.2001.0924
Ciociola, J. R. A. I., Querino, R. B., Zucchi, R. A., & Stouthamer, R. (2001). Molecular tool for identification of closely related species of Trichogramma (Hymenoptera: Trichogrammatidae): T. rojasi Nagaraja & T. lasallei Pinto. Neotropical Entomology, 30(4), 575-578. https://doi.org/10.1590/S1519-566X2001000400010 DOI: https://doi.org/10.1590/S1519-566X2001000400010
Ercan, F. S., Öztemiz, S., Tunçbilek, A., & Stouthamer, R. (2011). Sequence Analysis ofthe Ribosomal DNA ITS2 Regionin Two Trichogramma Species (Hymenoptera: Trichogrammatidae). Archives of Biological Sciences, 63(4), 949-954. https://doi.org/10.2298/ABS1104949E DOI: https://doi.org/10.2298/ABS1104949E
Ercan, F. S., Öztemiz, S., & Tunçbilek, A. (2013). Mitochondrial and ribosomal DNAsequence analysis for discrimination of Trichogramma euproctidis Girault and Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae). Turkish Journal of Entomology, 37(2), 195-201.
Ercan, F. S., Ateş, M. A., & Öztemiz, S. (2022). RDNA‑ITS2 characterization of Trichogramma species (Hymenoptera: Trichogrammatidae) in Turkey.Egyptian Journal of Biological Pest Control, 32, 50. https://doi.org/10.1186/s41938-022-00549-z. DOI: https://doi.org/10.1186/s41938-022-00549-z
Kimura, M. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111-120. https://doi.org/10.1007/BF01731581 PMid:7463489 DOI: https://doi.org/10.1007/BF01731581
Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), 1870-1874. https://doi.org/10.1093/molbev/msw054 PMid:27004904 PMCid: PMC8210823 DOI: https://doi.org/10.1093/molbev/msw054
Li-Ying, L. (1994). Worldwide use of Trichogramma for biological control of different crops: A survey. In E. Wajnberg and S. A. Hassan (eds.). Biological control with egg parasitoids. CAB International.
Miura, K., Matsuda, S., & Murai, T. (1990). Esterase zymograms of Trichogramma chilonis Ishii and T. ostriniae Pang et Chen (Hymenoptera, Trichogrammatidae). Japanese Journal of Entomology, 58(4), 689-692.
Monti, M. M., Nappo, A. G., & Giorgini, M. (2005). Molecular characterization of closely related species in the parasitic genus Encarsia (Hymenoptera: Aphelinidae) based on the mitochondrial cytochrome oxidase subunit I gene. Bulletin of Entomological Research, 95(5), 401-408. https://doi.org/10.1079/BER2005371 PMid:16197560 DOI: https://doi.org/10.1079/BER2005371
Nagarkatti, S., & Nagaraja, H. (1971). Redescription of some known species of Trichogramma, showing the importance of male genitalia as a diagnostic character. Bulletin of Entomological Research, 61, 13-31. https://doi.org/10.1017/S0007485300057412 DOI: https://doi.org/10.1017/S0007485300057412
Pinto, J. D., & Stouthamer, R. (1994). Systematics of the Trichogrammatidae with emphasis on Trichogramma. In E. Wajnberg and S. A. Hassan (eds.). Biological Control with Egg Parasitoids. CAB International Wallingford.
Pinto, J. D. (1999). Systematics of North American species of Trichogramma Westwood (Hymenoptera: Trichogrammatidae). Memoirs of the Entomological Society of Washington, 22, 1-287.
Pintureau, B. (1993). Enzymatic analysis of the genus Trichogramma (Hym.: Trichogrammatidae) in Europe. BioControl, 38(3), 411-431. https://doi.org/10.1007/BF02374459 DOI: https://doi.org/10.1007/BF02374459
Pizzol, J., Khoualdia, O., Ferran, A., Chavigny, P., & Vanlerberghe-Masutti, F. (2005). A single molecular marker to distinguish between strains of Trichogramma cacoeciae. Biocontrol Science and Technology, 15(5), 527-531. https://doi.org/10.1080/09583150500088934 DOI: https://doi.org/10.1080/09583150500088934
Posada, D. (2008). JModelTest: phylogenetic model averaging. Molecular Biology and Evolution, 25(7),1253-1256. https://doi.org/10.1093/molbev/msn083 PMid:18397919 DOI: https://doi.org/10.1093/molbev/msn083
Rosen, D. (1986). The role of taxonomy in effective biological control programs. Agriculture, Ecosystems and Environment, 15,121-129. https://doi.org/10.1016/0167-8809(86)90085-X DOI: https://doi.org/10.1016/0167-8809(86)90085-X
Santa Lucia, J. (1998). A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics. Proceedings of the National Academy of Sciences, 95(4), 1460-1465. https://doi.org/10.1073/pnas.95.4.1460 PMid:9465037 PMCid: PMC19045 DOI: https://doi.org/10.1073/pnas.95.4.1460
Sappal, N. P., Jeng, R. S., Hubbes, M., & Liu, F. (1995). Restriction fragment length polymorphisms in polymerase chain reaction amplified ribosomal DNAs of three Trichogramma (Hymenoptera: Trichogrammatidae) species. Genome, 38(3), 419-25. https://doi.org/10.1139/g95-055 PMid:7557356 DOI: https://doi.org/10.1139/g95-055
Silva, I. M. M. S. (1999). Identification and evaluation of Trichogramma parasitoids for biological control [Doctoral Thesis]. Wageningen University.
Smith, S. M. (1996). Biological Control with Trichogramma advances, Successes, and Potential of Their Use. Annual Review of Entomology, 41, 375-406. https://doi.org/10.1146/annurev.en.41.010196.002111 PMid:15012334 DOI: https://doi.org/10.1146/annurev.ento.41.1.375
Stouthamer, R., Luck, R. F., & Hamilton, W. D. (1990). Antibiotics cause parthenogenetic Trichogramma to revert to sex. Proceedings of the National Academy of Sciences, 87, 2424-2427. https://doi.org/10.1073/pnas.87.7.2424 PMid:11607070 PMCid: PMC53701 DOI: https://doi.org/10.1073/pnas.87.7.2424
Stouthamer, R., Hu, J., van Kan, F. J. P. M., Platner, G. R., & Pinto, J. D. (1999). The utility of internally transcribed spacer 2 DNA sequences of the nuclear ribosomal gene for distinguishing sibling species of Trichogramma. BioControl, 43, 421-440. https://doi.org/10.1023/A:1009937108715 DOI: https://doi.org/10.1023/A:1009937108715
Sümer, F., Tunçbilek, A., Öztemiz, S., Pintureau, B., Jones, P. R., & Stouthamer, R. (2009). A molecular key to the common species of Trichogramma of the Mediterranean region. BioControl, 54(1), 617-624. https://doi.org/10.1007/s10526-009-9219-8 DOI: https://doi.org/10.1007/s10526-009-9219-8
Sümer, F., Özcan, S., Öztemiz, S., & Tunçbilek, A. (2010). Esterase variation andsome biological characteristics of two Turkish Trichogramma (Hymenoptera: Trichogrammatidae) populations. North-Western Journal of Zoology, 6(1), 47-53.
Thomson, L., Rundle, B. J., Carew, M. E., & Hoffmann, A. A. (2003). Identification and characterization of Trichogramma species from south-eastern using the internal transcribed spacer 2 (ITS2) region of the ribosomal gene complex. Entomologia Experimentalis et Applicata, 106(3), 235-240. https://doi.org/10.1046/j.1570-7458.2003.00029.x DOI: https://doi.org/10.1046/j.1570-7458.2003.00029.x
Vanlerberghe-Masutti, F. (1994). Molecular identification and phylogeny of parasitic wasp species (Hymenoptera: Trichogrammatidae) by mitochondrial DNA RFLP and RAPD markers. Insect Molecular Biology, 3(4), 229-37. https://doi.org/10.1111/j.1365-2583.1994.tb00171.x PMid:7704306 DOI: https://doi.org/10.1111/j.1365-2583.1994.tb00171.x
Zhu, Y. C., & Williams, L. (2002). Detecting the egg parasitoid Anaphes iole (Hymenoptera: Mymaridae) in Tarnished plant bug (Heteroptera: Miridae) eggs by using molecular approach. Annals of the Entomological Society of America, 95(3), 359-365. https://doi.org/10.1603/0013-8746(2002)095[0359:DTEPAI]2.0.CO;2 DOI: https://doi.org/10.1603/0013-8746(2002)095[0359:DTEPAI]2.0.CO;2
Wiedenmann, R. N. (2000). Introduction to Biological Control. Midwest Institute for Biological Control.
Downloads
Publicado
Como Citar
Edição
Secção
Licença
Direitos de Autor (c) 2025 Fahriye Sümer Ercan, Sevcan Öztemiz
Este trabalho encontra-se publicado com a Licença Internacional Creative Commons Atribuição 4.0.
O autor mantém os seus direitos de marca registada e de patente para qualquer processo ou procedimento dentro do artigo.
O autor mantém o direito de partilhar, distribuir, executar e comunicar publicamente o artigo publicado no SHILAP Revista de lepidopterología, com reconhecimento inicial da sua publicação no SHILAP Revista de lepidopterología.
O autor reserva-se o direito de fazer uma publicação posterior da sua obra, desde a utilização do artigo até à sua publicação num livro, desde que indique a sua publicação inicial no SHILAP Revista de lepidopterología.
Cada apresentação ao SHILAP Revista de lepidopterología deve ser acompanhada por uma aceitação dos direitos de autor e reconhecimento da autoria. Ao aceitá-los, os autores retêm os direitos autorais da sua obra e concordam que o artigo, se aceite para publicação pelo SHILAP Revista de lepidopterología, será licenciado para uso e distribuição sob uma licença "Creative Commons Attribution 4.0 International" (CC BY 4.0), que permite a terceiros partilhar e adaptar o conteúdo para qualquer fim, dando o devido crédito à obra original.
Pode consultar aqui a versão informativa e o texto legal da licença. A indicação da licença CC BY 4.0 deve ser expressamente indicada desta forma quando necessário.
A partir de 2022, o conteúdo da versão impressa e digital é licenciado sob uma licença de utilização e distribuição "Creative Commons Attribution 4.0 International" (CC BY 4.0), que permite a terceiros partilhar e adaptar o conteúdo para qualquer fim, dando o devido crédito à obra original.
O conteúdo anterior da revista foi publicado sob uma licença tradicional de direitos de autor; no entanto, o arquivo está disponível para acesso livre.
Ao utilizar o conteúdo do SHILAP Revista de lepidopterología publicado antes do ano 2022, incluindo figuras, tabelas ou qualquer outro material em formato impresso ou eletrónico pertencem aos autores dos artigos, os autores devem obter a autorização do detentor dos direitos de autor. As responsabilidades legais, financeiras e criminais a este respeito pertencem ao(s) autor(es).
Em aplicação do Princípio de Prioridade do Código Internacional de Nomenclatura Zoológica, nenhuma outra versão além da publicada pela editora pode ser depositada em repositórios, websites pessoais ou similares.
