Trophic specialization of two morphospecies Chloridea virescens (Fabricius, 1777) in the dark tobacco crop in Pinar del Río, Cuba (Lepidoptera: Noctuidae, Heliothinae)
DOI:
https://doi.org/10.57065/shilap.227Keywords:
Lepidoptera, Noctuidae, Heliothinae, taxonomy, morphological variability, genetic variability, gen COI, CubaAbstract
Through integrative taxonomic identification, two morphospecies of the genus Chloridea Duncan [& Westwood], 1841, that affect black tobacco in the province of Pinar del Río, Cuba, are described. Last instar larvae were collected in two populations with different eating habits: the first affecting the terminal bud of the stem and the second affecting the reproductive organs of tobacco. The larvae were characterized by cuticular coloration and ornamentation, as well as by 11 morphological characters of chaetotaxy. The confirmation of the molecular taxonomy was carried out by sequencing the mitochondrial gene for cytochrome oxidase I (mtCOI), identifying Chloridea virescens (Fabricius, 1777) as the only species for both populations, with morphological variability and
genetic divergence associated with the trophic niche, suggesting a sympatric speciation.
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CORTÉS, E. J., TUTA, L. T. & GARCÍA, D., 2019.– Análisis de la evolución de la industria tabacalera en Ciego de Ávila, Cuba.– Universidad & Ciencia, 8(1): 164-174.
CHO, S., MITCHELL, A., MITTER, C., REGIER, J., MATTHEWS, M. & ROBERTSON, R., 2008.– Molecular phylogenetics of Heliothine moths (Lepidoptera: Noctuidae: Heliothinae) with comments on the evolution of host range and pest status.– Systematic Entomology, 33: 581-594. DOI: https://doi.org/10.1111/j.1365-3113.2008.00427.x
DINCA, V., DAPPORTO, L. & VILA, R., 2011.– A combined genetic-morphometric analysis unravels the complex biogeographical history of Polyommatus icarus and Polyommatus celina Common Blue butterflies.– Molecular Ecology, 20(18): 3921-3935. DOI: https://doi.org/10.1111/j.1365-294X.2011.05223.x
DOYLE, J. J. & DOYLE, J. L., 1990.– Isolation of plant DNA from fresh tissue.– Focus, 12:13-15. DOI: https://doi.org/10.2307/2419362
FOLMER, O., BLACK, M., HOEH, W., LUTZ, R. & VRIJENHOEK, R., 1994.– DNA primers for amplification of mitocondrial cytocrome c oxidase subunit I from diverse metazoan invertebrates.– Molecular Biology and Biotechnology, 3(5): 294-299.
GILLIGAN, T. M. & PASSOA, S. C., 2014.– Key to frequently named lepidopteran larvae intercepted, or potentially encountered, at us ports.– Lepintercept: 35. http://idtools.org/id/leps/lepintercept/LepIntercept_LarvalKey.pdf.
HALLMAN, G., 1978.– Clave taxonómica para las especies de Heliothis (Lepidoptera: Noctuidae) en Colombia.– Revista Colombiana de Entomología, 4(3-4): 61-69. DOI: https://doi.org/10.25100/socolen.v4i3-4.10377
HAUSMANN, A., HASZPRUNAR, G. & HEBERT, P. D. N., 2011.– DNA Barcoding the Geometrid Fauna of Bavaria (Lepidoptera): Successes, surprises, and questions.– Plos One, 6(2): 1-9. DOI: https://doi.org/10.1371/journal.pone.0017134
MARTÍNEZ-ROSAS, R., ARAUJO-RUIZ, K., CAMBRÓN-CRISANTOS, J. M. & VEGA-ORTÍZ, H. E., 2016.– Caracterización morfológica y molecular de larvas de Grapholita packardi (Zeller, 1875) y Grapholita prunivora (Walsh, 1868) (Lepidoptera: Tortricidae) de importancia económica.– Entomología Mexicana, 3: 961-968.
MÉNDEZ, A., 2003.– Aspectos biológicos sobre Heliothis virescens (Fabricius) (Lepidoptera: Noctuidae) en la Empresa Municipal Agropecuaria “Antonio Guiteras” de la zona norte de la provincia de Las Tunas.– Revista de Fitosanidad, 7(3): 21-25.
MITCHELL, A. & GOPURENKO, D., 2016.– DNA barcoding the Heliothinae (Lepidoptera: Noctuidae) of Australia and utility of DNA barcodes for pest identification in Helicoverpa and relatives.– Plos One, 11(8): 1-18. DOI: https://doi.org/10.1371/journal.pone.0160895
OPPENHEIM, S. J., GOULD, F. & HOPPER, K. R., 2017.– The genetic architecture of ecological adaptation: intraspecific variation in host plant use by the lepidopteran crop pest Chloridea virescens.– Heredity, 120: 234-250. DOI: https://doi.org/10.1038/s41437-017-0016-3
PÉREZ, J. C. & SURIS, M., 2012.– Ciclo de vida y reproducción de Heliothis virescens (F.) (Lepidoptera: Noctuidae) sobre garbanzo.– Revista de Protección Vegetal, 27(2): 85-89.
POGUE, M. G., 2013.– Revised status of Chloridea Duncan [and Westwood, 1841], for the Heliothis virescens species group (Lepidoptera: Noctuidae: Heliothinae) based on morphology and three genes.– Systematic Entomology, 38: 523-542. DOI: https://doi.org/10.1111/syen.12010
RIVAS, A. & MARTÍNEZ, M. A., 2015.– Heliothis tergeminas (Felder y Rogenhofer), nueva especie plaga en el cultivo del tabaco en Cuba.– Revista Protección Vegetal, 30: 77-80.
RODRÍGUEZ, F. L., MARTÍNEZ, M. A. & GÓMEZ, J. F., 2018.– Parámetros biológicos y tabla de vida de dos poblaciones de Chloridea virescens (Fabricius) (Lepidoptera: Noctuidae) asociadas al tabaco negro.– Revista Protección Vegetal, 33(2): 1-7.
SANTOS-ZAMORANO, B., HUANCA-MAMANI, W. & VARGAS, H. A., 2017.– Genetic divergence of the pest moth Chloridea virescens (Noctuidae: Heliothinae) feeding on a newly documented host plant in the Atacama Desert of Northern Chile.– Journal of the Lepidopterists’ Society, 71(4): 274-278. DOI: https://doi.org/10.18473/lepi.71i4.a9
TAMURA, K., STECHER, G., PETERSON, D., FILIPSKI, A. & KUMAR, S., 2013.– MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.– Molecular Biology and Evolution, 30(12): 2725-2729. DOI: https://doi.org/10.1093/molbev/mst197
THOMPSON, J. D., HIGGINS, D. G. & GIBSON, T. J., 1994.– CLUSTAL W. Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice.– Nucleic Acids Research, 22: 4673-4680. DOI: https://doi.org/10.1093/nar/22.22.4673
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Copyright (c) 2021 F. L. Rodríguez-Espinosa, Y. Santana-Baños, J. Pacheco-Escobar, M. A. Martínez-Rivero, I. G. López Muraira, J. F. Gómez-Leyva
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