Two new species and two new record species of the pruinosella species group of genus Athrips Billberg, 1820 from China (Lepidoptera: Gelechiidae)

Two species, Athrips albimacula Oh & Li, sp. nov. and A. lingchuana Oh & Li, sp. nov., are described as new; two species, A. pruinosella (Lienig & Zeller, 1846) and A. spiraeae (Staudinger, 1871), are newly recorded for China. Images of adults and genitalia of the above four species are illustrated.


Introduction
The genus Athrips was established by Billberg (1820), with Phalaena mouffetella Linnaeus, 1758 as the type species. It is characterized by having a broad and short uncus covered with strong setae, a strongly curved long gnathos, a transtilla with strongly developed medial lobes and a saccus with Xshaped sclerotized support (Bidzilya & Li, 2009). Athrips species were united into eleven species groups based on the phylogenetic analysis and their phylogenetic relationships established in broad terms (Bidzilya, 2005). To date, 44 species are recorded in the Palaeartic Region (Bidzilya & Li, 2009;Junnilainen & Nupponen, 2010;Bidzilya & Nupponen, 2018). Bidzilya & Li (2009) reviewed 22 Athrips species of the Chinese fauna, belonging to seven species groups. The aim of this study is to study the pruinosella species group from China.
The pruinosella group is characterized by the forewing of most species relatively broad, and grayish black with indistinct black spots; in the male genitalia by the well-developed transtilla with digitate medial lobes; in the female genitalia by the sternite VIII with well-developed patches of honeycomb pattern and wrinkles, the ostium bursae with dorsomedial sclerite, and distal part of the ductus bursae with additional lateral sclerite (Bidzilya, 2005). Nine species of the pruinosella-group were described from China previously. In this paper we describe two new species and to newly record two species for China.

Materials and methods
Specimens were collected in China by using light traps. All the studied specimens, including the types, are deposited in the Insect Collection of Nankai University, Tianjin, China (NKU).
Morphological characteristics of adults and genitalia were observed under an Olympus SZX7 stereomicroscope and an Olympus CH30 microscope. Dissection and slide mounting of genitalia followed the methods introduced by Li (2002). Images of adults and genitalia were taken with Leica M205A and Leica DM750 microscopes respectively, coupled with a Leica Application Suite 4.2 software, and were manipulated subsequently in Adobe Photoshop CS. Diagnosis: This species is externally similar to A. ravida Bidzilya & Li, 2009 in male genitalia, but differs from the latter in the forewing with distinctly large cream spot at basal 3/5 of costal margin and dorsum respectively, and in the male genitalia by the rectangular uncus, and the width of the anteriorly rounded saccus 2.5 times of its length; in the latter species, the forewing lacks a costal and a dorsal spot; the uncus is trapezoid-shaped, and the width of the anteriorly obtuse saccus is 2 times of its length in the male genitalia.

Taxonomy
Description Adult ( Figure 1): Wingspan 14.0-15.0 mm. Head grayish black, with long grayish white scales on upper margin of compound eyes. Labial palpus: second segment dark brown, with cream scales at apex, at base and on inner surface; third segment black except white at base on inner surface. Antenna: Scape black; flagellum black with dark brown rings. Thorax and tegula dark grayish brown. Forewing dark brown, with two large cream spots: one spot at basal 3/5 of costal margin, another at dorsum opposite to costal spot; fringe grayish brown. Hindwing and fringe light grayish brown. Fore-and midleg dark brown on outer surface, grayish yellow on inner surface, tarsi dark brown with grayish yellow rings; femora of hindleg grayish yellow, tibia dark brown on outer surface, grayish yellow on inner surface, tarsi dark brown and grayish yellow on outer surface, grayish yellow on inner surface.
Female: Unknown. Distribution: China (Qinghai). Etymology: The specific name is derived from the Latin albus and macula, referring to the large cream spots at costal and dorsal 3/5 of the forewing. Diagnosis: This species is similar to A. patockai (Povolnx, 1979) in both appearance and male genitalia. It can be distinguished from the latter of the forewing grayish brown with indistinct markings and in the male genitalia by the rectangular uncus, the distal end of the valva is blunt, and the transtilla lobe shorter than 2 times of its width; while in the latter species, the forewing black with distinct markings (Bidzilya, 2005, figures 53-55) and the uncus is trapezoid, the distal end of the valva is sharp, and the transtilla lobe is longer than 3 times of its width (Bidzilya, 2005, figures 120-122). The female genitalia of this species is diagnostic by the ductus bursae with sclerotized part distinctly longer than the apophyses anteriores; while in A. patockai, the female genitalis ductus bursae with sclerotized part approximately equal to the apophyses anteriores (Bidzilya, 2005 tipped scales. Labial palpus grayish brown except rather pale on inner surface of second segment and on both inner and ventral surface of third segment, scales tipped with whitish gray; second segment about 1.5 times length of third segment. Antenna: scape dark brown; flagellum dark brown with grayish brown rings. Thorax and tegula grayish brown, with gray tipped scales. Forewing grayish brown, with grayish white tipped scales; indistinct dark brown spot at basal 1/3 and 2/3 of fold respectively; fringe grayish brown. Hindwing and fringe light grayish brown. Fore-and midlegs grayish brown, tibia grayish brown alternated with grayish yellow; femur and tibia of hindleg brownish yellow on outer surface, grayish yellow on inner surface, tarsi grayish brown with grayish yellow rings. Male genitalia (Figure 6): Uncus rectangular, anterior margin emarginated, posterior margin with long setae. Gnathos sickle-shaped, strongly curved at basal 2/5, apex pointed, slightly hooked. Tegumen with a large semicircular anterior emargination. Valva straight, subparallel from near base to apex; apex far exceeding top of uncus. Sacculus wide basally, narrowed towards apex, distally, slightly bent inward, pointed at apex. Transtilla lobes thumber-like, length shorter than 2 times of width. Saccus short and broad, width about twice of length. Phallus swollen basally, distal portion straight, tapered from distal 1/3 to apex.
Distribution: China (Shanxi). Etymology: The specific name is from the type locality.
Athrips pruinosella (Lienig & Zeller, 1846) (Figures 3, 7 (Staudinger, 1871), but slightly differs from the latter by the rather dark grayish-brown head, thorax and tegula (Figure 3), the L-shaped gnathos with the straight distal part more than 2/5 of the total length (Figure 7), and the triangular ostium bursae ( Figure 10); in the latter species, the head, thorax and tegula are rather pale grayish-brown (Figure 4), the straight distal part of the crescent-shaped gnathos is less than 1/3 of the total length (Figure 8), and the ostium bursae is conical (Figure 11).
According to Bidzilya (2005), A. pruinosella differs slightly in the phallus tapered only in its distal quarter whilst in A. spiraeae it is evenly tapered from the base to apex; the female genitalia could be separated by the shape of medially elongated lateral sclerites of sternite VIII and the length of sclerotized part of the ductus bursae.
Note: This species is newly recorded in China.
Diagnosis Adult (Figure 4): Wingspan 13.5-14.5 mm. Athrips spiraeae is diagnostic in the male genitalia by the sub-rectangular uncus, the crescent-shaped gnathos strongly curved at basal 2/3, the valva with apex exceeding tip of the uncus, the sacculus gradually narrowed towards beak-shaped apex, the digitate transtilla lobes, and the phallus evenly tapered to apex (Figure 8). Athrips spiraeae is characterized in the female genitalia by the trapezoid sternite VIII as long as the apophyses anteriores and with lateral patches of honeycomb pattern, the conical ostium bursae with a narrow sclerite, and the rounded corpus bursae with spinules on the inner wall and with a saddle-shaped signum (Figure 11).
Athrips spiraeae is similar to A. pruinosella internally and externally. The differences between them are stated in the diagnosis of the latter species.
Distribution: China (Shanxi), Russia, Kazakhstan, Ukraine. Note: This species is newly recorded in China.